The Montpellier Leishmania Collection, from a Laboratory Collection to a Biological Resource Center: A 39-Year-Long Story.
Biopreserv Biobank
; 14(6): 470-479, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27379470
ABSTRACT
We report the development of a laboratory collection of Leishmania that was initiated in 1975 and, after 39 years, has become an international Biological Resource Center (BRC-Leish, Montpellier, France, BioBank No. BB-0033-00052), which includes 6353 strains belonging to 36 Leishmania taxa. This is a retrospective analysis of the technical and organizational changes that have been adopted over time to take into account the technological advances and related modifications in the collection management and quality system. The technical improvements concerned the culture and cryopreservation techniques, strain identification by isoenzymatic and molecular techniques, data computerization and quality management to meet the changes in international standards, and in the cryogenic and microbiological safety procedures. The BRC is working toward obtaining the NF-S 96-900 certification in the coming years. Our long-term expertise in Leishmania storage and typing and collection maintenance should encourage field epidemiologists and clinical practitioners in endemic countries to secure their own strain collection with the help of the French BRC-Leish.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
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Bancos de Espécimes Biológicos
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Leishmania
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article