A proteomic and genetic analysis of the Neurospora crassa conidia cell wall proteins identifies two glycosyl hydrolases involved in cell wall remodeling.
Fungal Genet Biol
; 94: 47-53, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27381444
ABSTRACT
A proteomic analysis of the conidial cell wall identified 35 cell wall proteins. A comparison with the proteome of the vegetative hyphae showed that 16 cell wall proteins were shared, and that these shared cell wall proteins were cell wall biosynthetic proteins or cell wall structural proteins. Deletion mutants for 34 of the genes were analyzed for phenotypes indicative of conidial cell wall defects. Mutants for two cell wall glycosyl hydrolases, the CGL-1 ß-1,3-glucanase (NCU07523) and the NAG-1 exochitinase (NCU10852), were found to have a conidial separation phenotype. These two enzymes function in remodeling the cell wall between adjacent conidia to facilitate conidia formation and dissemination. Using promoterRFP and promoterGFP constructs, we demonstrated that the promoters for 15 of the conidia-specific cell wall genes, including cgl-1 and nag-1, provided for conidia-specific gene expression or for a significant increase in their expression during conidiation.
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Assunto principal:
Esporos Fúngicos
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Proteínas Fúngicas
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Parede Celular
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Glicosídeo Hidrolases
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Hexosaminidases
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Neurospora crassa
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article