The effect of Liuwei Dihuang decoction on PI3K/Akt signaling pathway in liver of type 2 diabetes mellitus (T2DM) rats with insulin resistance.
J Ethnopharmacol
; 192: 382-389, 2016 Nov 04.
Article
em En
| MEDLINE
| ID: mdl-27401286
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Liuwei Dihaung decoction (LWDHT) is a well-known classic traditional Chinese medicine formula, consists of six herbs including Rehmannia glutinosa Libosch.(family Scrophulariaceae), Cornus officinalis Sieb.(family Cornaceae), Dioscorea opposite Thunb.(family Dioscoreaceae), Alisma orientale(G. Samuelsson) Juz (family Alismataceae), Poria cocos (Schw.) Wolf (family Polyporaceae) and Paeonia suffruticosa Andrews (family Paeoniaceae). It has been used in the treatment of many types of diseases with signs of deficiency of Yin in the kidneys in China clinically. This study is aimed at investigating the effect of Liuwei dihuang decoction on PI3K/Akt signaling pathway in liver of T2DM rats with insulin resistance. MATERIALS AND METHODS:
T2DM model was induced in male Sprague-Dawley (SD) rats by high sugar and high fat diets combined with small dose of streptozocin (STZ) injection. The successful T2DM rats were randomly allocated three group--vehicle group, positive control group and Liuwei Dihuang decoction group. After 12-weeks treatment with distilled water, rosiglitazone and LWDHT by intragastric administration respectively, the rats were put to death in batches. The variance of fasting blood glucose (FBG) and fasting insulin (FINS) in serum were determined, the pathological changes of each rats' liver were observed by hematoxylin-eosin (HE) staining, the expression of insulin receptor substrate 2(IRS2), phosphatidylinositol 3-kinase (PI3K) and protein kinas B (Akt) involving the canonical PI3K/Akt signaling pathway were detected by Real-time fluorescent quantitative PCR (RT-PCR), and the expression level of IRS2, PI3K, Akt protein and phosphorylated IRS2, PI3K, Akt protein were evaluated by Western Blot. All the data were analyzed by SPSS 17.0.RESULTS:
Four weeks of treatment with LWDHT could significantly decrease the level of FBG and FINS in serum, improve the cellular morphology of liver, kidney, pancreas tissue, and the expression of IRS2, PI3K, Akt mRNA and phosphorylated IRS2, PI3K, Akt protein involved in the canonical PI3K/Akt signaling pathway of T2DM rats in liver were significantly up-regulated, while the total IRS2, PI3K, and Akt protein had no obvious changes.CONCLUSIONS:
The results suggest that Liuwei Dihuang decoction could intervene insulin resistance of T2DM, in part, through regulation of canonical PI3K/Akt signaling pathway of T2DM rats in liver.Palavras-chave
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MEDLINE
Assunto principal:
Glicemia
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Resistência à Insulina
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Medicamentos de Ervas Chinesas
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Diabetes Mellitus Experimental
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Diabetes Mellitus Tipo 2
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Proteínas Proto-Oncogênicas c-akt
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Fosfatidilinositol 3-Quinase
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Hipoglicemiantes
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Fígado
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article