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Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions.
Bouchet, Benjamin P; Gough, Rosemarie E; Ammon, York-Christoph; van de Willige, Dieudonnée; Post, Harm; Jacquemet, Guillaume; Altelaar, Af Maarten; Heck, Albert Jr; Goult, Benjamin T; Akhmanova, Anna.
Afiliação
  • Bouchet BP; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
  • Gough RE; School of Biosciences, University of Kent, Canterbury, United Kingdom.
  • Ammon YC; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
  • van de Willige D; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
  • Post H; Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, The Netherlands.
  • Jacquemet G; Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.
  • Altelaar AM; Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
  • Heck AJ; The Netherlands Proteomics Centre, Utrecht University, Utrecht, The Netherlands.
  • Goult BT; Turku Centre for Biotechnology, University of Turku, Turku, Finland.
  • Akhmanova A; Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, The Netherlands.
Elife ; 52016 07 13.
Article em En | MEDLINE | ID: mdl-27410476
The cross-talk between dynamic microtubules and integrin-based adhesions to the extracellular matrix plays a crucial role in cell polarity and migration. Microtubules regulate the turnover of adhesion sites, and, in turn, focal adhesions promote the cortical microtubule capture and stabilization in their vicinity, but the underlying mechanism is unknown. Here, we show that cortical microtubule stabilization sites containing CLASPs, KIF21A, LL5ß and liprins are recruited to focal adhesions by the adaptor protein KANK1, which directly interacts with the major adhesion component, talin. Structural studies showed that the conserved KN domain in KANK1 binds to the talin rod domain R7. Perturbation of this interaction, including a single point mutation in talin, which disrupts KANK1 binding but not the talin function in adhesion, abrogates the association of microtubule-stabilizing complexes with focal adhesions. We propose that the talin-KANK1 interaction links the two macromolecular assemblies that control cortical attachment of actin fibers and microtubules.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Talina / Adesões Focais / Proteínas Supressoras de Tumor / Microtúbulos Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Talina / Adesões Focais / Proteínas Supressoras de Tumor / Microtúbulos Idioma: En Ano de publicação: 2016 Tipo de documento: Article