Simultaneous assessment of endogenous thiol compounds by LC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1029-1030: 213-221, 2016 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-27442797
ABSTRACT
Biological thiol compounds are very important molecules that participate in various physiological events. Alteration in levels of endogenous thiols has been suggested as a biomarker of early stage of pathological changes. We reported a chemical derivatization- and LC-MS/MS-based approach to simultaneously determine thiol compounds including glutathione (GSH), cysteine (Cys), N-acetyl cysteine (NAC), homocysteine (Hcy), and cysteinylglycine (CysGly) in biological samples. Thiol-containing samples were derivatized with monobromobimane (mBrB) at room temperature, followed by LC-MS/MS analysis. Assessment of the analytes with baseline separation was completed within 10min, using a gradient elution on a C18 reversed-phase column. Excellent linearity was observed for all analytes over their concentration ranges of 1-400µM. The lowest limits of detection (S/N=3) in a range from 0.31fmol (for NAC) to 4.98fmol (for CysGly) were achieved. The results indicate that this approach was sensitive, selective, and well suited for high-throughput quantitative determination of the biological thiols.
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Acetilcisteína
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Cromatografia Líquida de Alta Pressão
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Cisteína
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Dipeptídeos
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Glutationa
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En
Ano de publicação:
2016
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Article