[Correlation between MALDI-TOF Vitek-MSTM system and conventional identification methods of gastrointestinal infection causing bacteria]. / Correlación entre el sistema de MALDI-TOF Vitek-MSTM y los métodos convencionales de identificación de bacterias causantes de infección gastrointestinal.
Rev Esp Quimioter
; 29(5): 265-8, 2016 Oct.
Article
em Es
| MEDLINE
| ID: mdl-27454012
ABSTRACT
OBJECTIVE:
Rapid identification of pathogens is essential for the diagnosis of gastrointestinal infections. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has shown to be effective and fast for the identification of microorganisms. The objective of this study was to evaluate the correlation between Vitek-MSTM and conventional methods for bacterial identification causing gastrointestinal infection.METHODS:
A total of 329 gastrointestinal pathogens were identified using Vitek-MSTM (v2 SARAMIS MS -ID, bioMérieux, Marcy-I´Étoile, France) and routine diagnostic methods simultaneously. In cases of discrepancy 16SrRNA gene sequencing was performed.RESULTS:
The correlation between Vitek-MSTM and diagnostic methods was 100% except for Yersinia enterocolitica (94.1%), Helicobacter pylori (10%) and Aeromonas veronii (0 %).CONCLUSIONS:
Vitek-MSTM is a quick and useful method for identification of enterophatogenic bacteria. It is necessary to improve the performance of the system for the identification of H. pylori and A. veronii.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Gastroenteropatias
/
Infecções
Idioma:
Es
Ano de publicação:
2016
Tipo de documento:
Article