Protective effects of miR-29a on diabetic glomerular dysfunction by modulation of DKK1/Wnt/ß-catenin signaling.
Sci Rep
; 6: 30575, 2016 07 27.
Article
em En
| MEDLINE
| ID: mdl-27460630
ABSTRACT
Dysregulation of specific microRNAs or Wnt/ß-catenin signaling pathway is critically implicated in the pathogenesis of various renal diseases. However, the relationship between microRNAs and Wnt/ß-catenin signaling in diabetes-induced glomerular sclerosis remains unknown. Here, we found that decreased miR-29a expression and attenuated Wnt/ß-catenin signaling were concomitantly detected in glomeruli of streptozotocin-induced diabetic mice. Gain of miR-29a function in diabetic mice substantially increased the expression of ß-catenin and blocked the expressions of profibrotic gene markers, including DKK1 (a Wnt antagonist), TGF-ß1 and fibronectin, in glomerular mesangium. Moreover, in the normal mice treated with miR-29a inhibitor, renal fibrosis was induced with an attenuated Wnt/ß-catenin signaling activity. Consistently, the constructed miR-29a transgenic mice that supported sustained Wnt/ß-catenin signaling had the ability to block the expressions of profibrotic genes after induction of diabetes. We also demonstrated that miR-29a acts as a positive regulator of Wnt/ß-catenin signaling in cultured mesangial cells and functions to protect cell apoptosis and fibrosis. Importantly, we showed that activation of Wnt/ß-catenin signaling in cultured mesangial cells by transfecting the ß-catenin (Δ45) mutant or by a GSK-3ß inhibitor reversely upregulated miR29a. Our findings suggest that the reciprocal relationship between miR-29a and DKK1/Wnt/ß-catenin signaling may play an important part in protecting renal fibrogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos e Proteínas de Sinalização Intercelular
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MicroRNAs
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Diabetes Mellitus Experimental
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Glomérulos Renais
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article