ß-(1â3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
Int J Med Mushrooms
; 18(4): 313-20, 2016.
Article
em En
| MEDLINE
| ID: mdl-27481297
ABSTRACT
Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction. The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a ß-glucan with glycosidic links of the (1â3)-ß type and most likely 4-O substituted. In addition, the biological effect of the ß-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice. Biological assays were assessed for toxicity and cell activation, interleukin-6 cytokine concentrations, and the ability to stimulate phagocytic activity. There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide. The results indicate that a ß-(1â3)-glucan isolated from G. australe can be classified as a biological response modifier.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fagocitose
/
Polissacarídeos
/
Interleucina-6
/
Ganoderma
/
Beta-Glucanas
/
Fatores Imunológicos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article