A shared N-terminal hydrophobic tail for the formation of nanoparticulates.
Nanomedicine (Lond)
; 11(17): 2289-303, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27499052
ABSTRACT
AIM:
Nanoparticulate design is important for the production of nanotechnological materials and passive immunogens. Using lessons from our hepatitis E vaccine, we herein design protein-based nanoparticles through incorporation of an N-terminal hydrophobic tail (NHT, located on HEV ORF2 aa368-460). MATERIALS &METHODS:
Flu HA1, HIV gp41/gp120/p24, HBsAg and HPV16 L2 were fused with NHT, expressed in Escherichia coli and subjected to self-assembly in vitro. Nanosized particles were characterized by size-exclusion chromatography and negative electron microscopy. Immunogenicity was assessed in mice.RESULTS:
All the NHT-fused proteins spontaneously formed nanoparticulates and presented with immunogenicity approximately 2-log over their nonassembling forms.CONCLUSION:
Protein self-assembly provides an attractive means to create nanosized particles that bear specific antigens. Our strategy outlines a novel and shared method for the design of immunogenic nanoparticles.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Vírus
/
Viroses
/
Vacinas Virais
/
Nanopartículas
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article