Direct glycan structure determination of intact N-linked glycopeptides by low-energy collision-induced dissociation tandem mass spectrometry and predicted spectral library searching.

Pai, Pei-Jing; Hu, Yingwei; Lam, Henry

Pai, Pei-Jing; Hu, Yingwei; Lam, Henry.

Afiliação

Pai PJ; Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong; Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung, Taiwan.
Hu Y; Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Lam H; Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong; Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. Electronic address: kehlam@ust.hk.

Anal Chim Acta ; 934: 152-62, 2016 Aug 31.

Article em En | MEDLINE | ID: mdl-27506355

Assuntos

Glicopeptídeos/química; Polissacarídeos/análise; Glicopeptídeos/metabolismo; Humanos; Biblioteca de Peptídeos; Espectrometria de Massas em Tandem; Tripsina/metabolismo

Palavras-chave

Low-energy collision-induced dissociation tandem mass spectrometry; N-glycopeptide identification; Predicted spectral library searching; Site-specific glycosylation analysis

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Glicopeptídeos Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo

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PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / Glicopeptídeos Idioma: En Ano de publicação: 2016 Tipo de documento: Article