Enhancement of astaxanthin production from Haematococcus pluvialis mutants by three-stage mutagenesis breeding.
J Biotechnol
; 236: 71-7, 2016 Oct 20.
Article
em En
| MEDLINE
| ID: mdl-27527395
ABSTRACT
Haematococcus pluvialis was modified for higher astaxanthin production compatible with the superiorities of high biomass and high activity by three-stage mutagenesis breeding. UV irradiation mutants named UV11-4 made an increase on cell dry weight, but showed a longer growth circle than the wild type. On the basis of UV mutants, ethyl methane sulphonate (EMS) mutants E2-5 cut down the latent phase, brought forward and extended the logarithmic phase. The inhibitor diphenylamine (DPA) was employed to screen high-yield astaxanthin producer by the color change of colonies from green to red on solid medium. Via the contravariant cultivation, proliferation and transformation, the mutant DPA12-2 possessed an 1.7-fold astaxanthin production compared to the wild type, reaching 47.21±3.30mg/g dry cells.
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MEDLINE
Assunto principal:
Mutagênese
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Clorófitas
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Engenharia Metabólica
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article