Rapid and sensitive quantitation of heme in hemoglobinized cells.

Marcero, Jason R; Piel Iii, Robert B; Burch, Joseph S; Dailey, Harry A

Marcero, Jason R; Piel Iii, Robert B; Burch, Joseph S; Dailey, Harry A.

Afiliação

Marcero JR; Department of Biochemistry and Molecular Biology, Biomedical and Health Sciences Institute, University of Georgia, Athens.
Piel Iii RB; Department of Biochemistry and Molecular Biology, Biomedical and Health Sciences Institute, University of Georgia, Athens.
Burch JS; Department of Biochemistry and Molecular Biology, Biomedical and Health Sciences Institute, University of Georgia, Athens.
Dailey HA; Department of Biochemistry and Molecular Biology, Biomedical and Health Sciences Institute, University of Georgia, Athens.

Biotechniques ; 61(2): 83-91, 2016.

Article em En | MEDLINE | ID: mdl-27528073

Assuntos

Heme/análise; Heme/química; Hemoglobinas/química; Espectrometria de Fluorescência/métodos; Animais; Linhagem Celular Tumoral; Desenho de Equipamento; Índices de Eritrócitos; Feminino; Heme/análogos & derivados; Hemoglobinas/análise; Humanos; Células K562; Limite de Detecção; Modelos Lineares; Masculino; Camundongos; Reprodutibilidade dos Testes

Palavras-chave

K562 cells; fluorescence heme assay; heme; hemoglobin; in-situ spectroscopy; integrating cavity; murine erythroleukemia cells; pyridine hemochromogen assay

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Fluorescência / Hemoglobinas / Heme Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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