[Differentiation of K562 Cells Induced by Pulsatilla Saponin A into Erythroid Lineage].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 24(4): 975-9, 2016 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-27531759
ABSTRACT
OBJECTIVE:
To explore the differentiation-inducing potentiality of Pulsatilla saponin A on K562 cells.METHODS:
Pulsatilla saponin A of different concentrations was used to treat K562 cells; the benzidine staining and the hemoglobinometry were applied to measure the change of hemoglobin content; the flow cytometry (FCM) was used to detect the expression of CD71 and GPA on K562 cells.RESULTS:
K562 cells treated with 4 µg/ml pulsatilla saponin A differentiated into the erythroid lineage. With the treatment of pulsatilla saponin A, the hemoglobin content in K562 cells increased significantly; CD71 and GPA expression on the K562 cell surface were up-regulated.CONCLUSION:
Pulsatilla saponin A can induce K562 cells to differentiate into erythroid lineage.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Linhagem da Célula
/
Células Eritroides
Idioma:
Zh
Ano de publicação:
2016
Tipo de documento:
Article