Production of Conidia by the Fungus Metarhizium anisopliae Using Solid-State Fermentation.
Methods Mol Biol
; 1477: 61-9, 2016.
Article
em En
| MEDLINE
| ID: mdl-27565492
ABSTRACT
This chapter describes the production of conidia by Metarhizium anisopliae using solid-state fermentation. Before production of conidia, procedures for strains conservation, reactivation, and propagation are essential in order to provide genetic stability of the strains. The strain is conserved in freeze-dried vials and then reactivated through insect inoculation. Rice is used as a substrate for the conidia production in two different bioreactors plastic bags and tubular bioreactor. The CO2 production in the tubular bioreactors is measured with a respirometer; this system allows calculating indirect growth parameters as lag time (tlag) (25-35 h), maximum rate of CO2 production (rCO2 max) (0.5-0.7 mg/gdm h), specific rate of CO2 production (µ) (0.10-0.15 1/h), and final CO2 production (CO2) (100-120 mg/gdm). Conidial yield per gram of dry substrate (gdm) should be above 1 × 10(9) conidia/gdm after 10 days of incubation. Germination and viability of conidia obtained after 10 days of incubation should be above 80 % and 75 %, respectively. Bioassays using of Tenebrio molitor as a host insect should yield a final mortality above 80 %.
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Base de dados:
MEDLINE
Assunto principal:
Esporos Fúngicos
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Metarhizium
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Fermentação
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Agentes de Controle Biológico
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article