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Ebola Virus Disease Diagnostics, Sierra Leone: Analysis of Real-time Reverse Transcription-Polymerase Chain Reaction Values for Clinical Blood and Oral Swab Specimens.
Erickson, Bobbie R; Sealy, Tara K; Flietstra, Tim; Morgan, Laura; Kargbo, Brima; Matt-Lebby, Victor E; Gibbons, Aridth; Chakrabarti, Ayan K; Graziano, James; Presser, Lance; Flint, Mike; Bird, Brian H; Brown, Shelley; Klena, John D; Blau, Dianna M; Brault, Aaron C; Belser, Jessica A; Salzer, Johanna S; Schuh, Amy J; Lo, Michael; Zivcec, Marko; Priestley, Rachael A; Pyle, Meredith; Goodman, Christin; Bearden, Scott; Amman, Brian R; Basile, Alison; Bergeron, Éric; Bowen, Michael D; Dodd, Kimberly A; Freeman, Molly M; McMullan, Laura K; Paddock, Christopher D; Russell, Brandy J; Sanchez, Angela J; Towner, Jonathan S; Wang, David; Zemtsova, Galina E; Stoddard, Robyn A; Turnsek, Maryann; Guerrero, Lisa Wiggleton; Emery, Shannon L; Stovall, Janae; Kainulainen, Markus H; Perniciaro, Jamie L; Mijatovic-Rustempasic, Slavica; Shakirova, Gulchekhra; Winter, Jörn; Sexton, Christopher; Liu, Feng.
Afiliação
  • Erickson BR; Viral Special Pathogens Branch.
  • Sealy TK; Viral Special Pathogens Branch.
  • Flietstra T; Viral Special Pathogens Branch.
  • Morgan L; Viral Special Pathogens Branch.
  • Kargbo B; Ministry of Health and Sanitation, Freetown, Sierra Leone.
  • Matt-Lebby VE; Ministry of Health and Sanitation, Freetown, Sierra Leone.
  • Gibbons A; Viral Special Pathogens Branch.
  • Chakrabarti AK; Viral Special Pathogens Branch.
  • Graziano J; Bacterial Special Pathogens Branch.
  • Presser L; Viral Special Pathogens Branch.
  • Flint M; Viral Special Pathogens Branch.
  • Bird BH; Viral Special Pathogens Branch.
  • Brown S; Viral Special Pathogens Branch.
  • Klena JD; Division of Global Health Protection, CDC, Beijing, China.
  • Blau DM; Infectious Diseases Pathology Branch.
  • Brault AC; Arboviral Diseases Branch.
  • Belser JA; Influenza Division, Immunology and Pathogenesis Branch.
  • Salzer JS; Pox and Rabies Branch.
  • Schuh AJ; Viral Special Pathogens Branch.
  • Lo M; Viral Special Pathogens Branch.
  • Zivcec M; Viral Special Pathogens Branch.
  • Priestley RA; Rickettsial Zoonoses Branch.
  • Pyle M; Division of Blood Disorders.
  • Goodman C; Arboviral Diseases Branch.
  • Bearden S; Bacterial Diseases Branch, CDC, Fort Collins, Colorado.
  • Amman BR; Viral Special Pathogens Branch.
  • Basile A; Arboviral Diseases Branch.
  • Bergeron É; Viral Special Pathogens Branch.
  • Bowen MD; Gastroenteritis and Respiratory Virus Laboratory Branch.
  • Dodd KA; School of Veterinary Medicine, University of California-Davis.
  • Freeman MM; Enteric Diseases Laboratory Branch.
  • McMullan LK; Viral Special Pathogens Branch.
  • Paddock CD; Rickettsial Zoonoses Branch.
  • Russell BJ; Arboviral Diseases Branch.
  • Sanchez AJ; Office of Technology and Innovation.
  • Towner JS; Viral Special Pathogens Branch.
  • Wang D; Influenza Division, Immunology and Pathogenesis Branch.
  • Zemtsova GE; Rickettsial Zoonoses Branch.
  • Stoddard RA; Bacterial Special Pathogens Branch.
  • Turnsek M; Enteric Diseases Laboratory Branch.
  • Guerrero LW; Viral Special Pathogens Branch.
  • Emery SL; Influenza Division, Virology, Surveillance, and Diagnosis Branch.
  • Stovall J; Arboviral Diseases Branch.
  • Kainulainen MH; Viral Special Pathogens Branch.
  • Perniciaro JL; Rickettsial Zoonoses Branch.
  • Mijatovic-Rustempasic S; Gastroenteritis and Respiratory Virus Laboratory Branch.
  • Shakirova G; Inorganic and Radiation Analytical Toxicology Branch.
  • Winter J; Influenza Division, Virology, Surveillance, and Diagnosis Branch.
  • Sexton C; Bacterial Diseases Branch, CDC, Fort Collins, Colorado.
  • Liu F; Influenza Division, Immunology and Pathogenesis Branch.
J Infect Dis ; 214(suppl 3): S258-S262, 2016 10 15.
Article em En | MEDLINE | ID: mdl-27587631
During the Ebola virus outbreak of 2013-2016, the Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis of the B2M endogenous control Ct values showed its utility in monitoring specimen quality, comparing results with different specimen types, and interpretation of results. For live patients, blood is the most sensitive specimen type and oral swabs have little diagnostic utility. However, swabs are highly sensitive for diagnostic testing of corpses.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Viral / Surtos de Doenças / Doença pelo Vírus Ebola / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Ebolavirus Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Viral / Surtos de Doenças / Doença pelo Vírus Ebola / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Ebolavirus Idioma: En Ano de publicação: 2016 Tipo de documento: Article