Chlamydia pneumoniae CPj0783 interaction with Huntingtin-protein14.
Int Microbiol
; 18(4): 225-33, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-27611675
ABSTRACT
Chlamydia pneumoniae is a Gram-negative, obligate intracellular pathogen that causes community-acquired respiratory infections. After C. pneumoniae invades host cells, it disturbs the vesicle transport system to escape host lysosomal or autophagosomal degradation. By using a yeast mis-sorting assay, we found 10 C. pneumoniae candidate genes involved in aberrant vesicular trafficking in host cells. One of the candidate genes, CPj0783, was recognized by antibodies from C. pneumoniae-infected patients. The expression of CPj0783 was detected at mid to late-cycle time points and increased during the inclusion maturation. Two-hybrid screening in yeast cells revealed that CPj0783 interacted with Huntingtin-interacting protein 14 (HIP14). The specific interaction between CPj0783 and HIP14 could be demonstrated by an in vivo co-immunoprecipitation assay and an in vitro GST pull-down assay. It was also demonstrated that HIP14 was localized in the Golgi apparatus and colocalized with CPj0783. HIP14 has a palmitoyl transferase activity that is involved in the palmitoylation-dependent vesicular trafficking of several acylated proteins. These findings suggest that CPj0783 might cause abnormal vesicle-mediated transport by interacting with HIP14. [Int Microbiol 18(4)225-233 (2015)].
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MEDLINE
Assunto principal:
Proteínas de Bactérias
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Aciltransferases
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Chlamydophila pneumoniae
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Infecções por Chlamydophila
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Proteínas Adaptadoras de Transdução de Sinal
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Proteínas do Tecido Nervoso
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article