The vesicular stomatitis virus matrix protein inhibits NF-κB activation in mouse L929 cells.
Virology
; 499: 99-104, 2016 12.
Article
em En
| MEDLINE
| ID: mdl-27643886
ABSTRACT
A previous study found that NF-κB activation is delayed in L929 cells infected with wild-type (wt) strains of VSV, while activation occurred earlier in cells infected with mutant strain T1026R1 (R1) that encodes a mutation in the cytotoxic matrix (M) protein. The integrity of the other R1 proteins is unknown; therefore our goal was to identify the viral component responsible for preventing NF-κB activation in L929 cells. We found that the M protein inhibits viral-mediated activation of NF-κB in the context of viral infection and when expressed alone via transfection, and that the M51R mutation in M abrogates this function. Addition of an IκB kinase (IKK) inhibitor blocked NF-κB activation and interferon-ß mRNA expression in cells infected with viruses encoding the M51R mutation in M. These results indicate that the VSV M protein inhibits activation of NF-κB by targeting an event upstream of IKK in the canonical pathway.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Matriz Viral
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NF-kappa B
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Vírus da Estomatite Vesicular Indiana
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article