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Designing monoclonal antibody fragment-based affinity resins with high binding capacity by thiol-directed immobilisation and optimisation of pore/ligand size ratio.
Sakhnini, Laila Ismail; Pedersen, Anja Kallesøe; Ahmadian, Haleh; Hansen, Jens-Jacob; Bülow, Leif; Dainiak, Maria.
Afiliação
  • Sakhnini LI; Department of Downstream Technologies, Novo Nordisk A/S, Novo Nordisk Park, 2760 Måløv, Denmark; Department of Pure and Applied Biochemistry, Lund University, Naturvetarvägen 14, 223 62 Lund, Sweden. Electronic address: llsh@novonordisk.com.
  • Pedersen AK; Department of Biopharm Purification Dev. & Virology, Novo Nordisk A/S, Hagedornsvej 1, 2820 Gentofte, Denmark.
  • Ahmadian H; New affiliation, CMC Biologics A/S, Vandtårnsvej 83, 2860 Søborg, Denmark.
  • Hansen JJ; Department of Expression Technologies, Novo Nordisk A/S, Novo Nordisk Park, 2760 Måløv, Denmark.
  • Bülow L; Department of Pure and Applied Biochemistry, Lund University, Naturvetarvägen 14, 223 62 Lund, Sweden.
  • Dainiak M; Department of Downstream Technologies, Novo Nordisk A/S, Novo Nordisk Park, 2760 Måløv, Denmark.
J Chromatogr A ; 1468: 143-153, 2016 Oct 14.
Article em En | MEDLINE | ID: mdl-27663728
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sefarose / Compostos de Sulfidrila / Técnicas de Química Analítica / Ligantes / Resinas de Troca Aniônica / Anticorpos Monoclonais Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sefarose / Compostos de Sulfidrila / Técnicas de Química Analítica / Ligantes / Resinas de Troca Aniônica / Anticorpos Monoclonais Idioma: En Ano de publicação: 2016 Tipo de documento: Article