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One-Step Purification of Melittin Derived from Apis mellifera Bee Venom.
Teoh, Angela Ching Ling; Ryu, Kyoung-Hwa; Lee, Eun Gyo.
Afiliação
  • Teoh ACL; Korea University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
  • Ryu KH; Biotechnology Process Engineering Centre, KRIBB, Cheongju 28116, Republic of Korea.
  • Lee EG; Korea University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
J Microbiol Biotechnol ; 27(1): 84-91, 2017 Jan 28.
Article em En | MEDLINE | ID: mdl-27666985
The concern over the use of melittin in honey bee venom due to its adverse reaction caused by allergens such as phospholipase A2 (PLA2) and hyaluronidase (HYA) has been an obstacle towards its usage. We developed a novel single-step method for melittin purification and the removal of PLA2 and HYA. This study explores the influence of pH, buffer compositions, salt concentration, and types of cation-exchange chromatography resins on the recovery of melittin and the removal of both HYA and PLA2. Melittin was readily purified with a strong cation-exchange resin at pH 6.0 with sodium phosphate buffer. It resulted in a recovery yield of melittin up to 93% (5.87 mg from a total of 6.32 mg of initial melittin in crude bee venom), which is higher than any previously reported studies on melittin purification. PLA2 (99%) and HYA (96%) were also successfully removed. Our study generates a single-step purification method for melittin with a high removal rate of PLA2 and HYA, enabling melittin to be fully utilized for its therapeutic purposes.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2017 Tipo de documento: Article