Hybrid Minimal Core Streptavidin-Obelin as a Versatile Reporter for Bioluminescence-based Bioassay.
Photochem Photobiol
; 93(2): 548-552, 2017 03.
Article
em En
| MEDLINE
| ID: mdl-27696426
ABSTRACT
Ca2+ -regulated photoprotein obelin was genetically fused with a minimum-sized core streptavidin. Hybrid protein (SAV-OL) was produced by bacterial expression and applied as a specific bioluminescent probe in diverse solid-phase assays. The obtained results clearly demonstrate specific activity of each domain indicating its proper folding with favorable space orientation. SAV-OL has been shown to be a much more sensitive label than the chemical conjugate of a full-length streptavidin with obelin.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genes Reporter
/
Estreptavidina
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Luminescência
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Proteínas Luminescentes
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article