Multivesicular body formation enhancement and exosome release during endoplasmic reticulum stress.
Biochem Biophys Res Commun
; 480(2): 166-172, 2016 11 11.
Article
em En
| MEDLINE
| ID: mdl-27725157
ABSTRACT
The endoplasmic reticulum (ER) plays a pivotal role in maintaining cellular homeostasis. However, numerous environmental and genetic factors give rise to ER stress by inducing an accumulation of unfolded proteins. Under ER stress conditions, cells initiate the unfolded protein response (UPR). Here, we demonstrate a novel aspect of the UPR by electron microscopy and immunostaining analyses, whereby multivesicular body (MVB) formation was enhanced after ER stress. This MVB formation was influenced by inhibition of ER stress transducers inositol required enzyme 1 (IRE1) and PKR-like ER kinase (PERK). Furthermore, exosome release was also increased during ER stress. However, in IRE1 or PERK deficient cells, exosome release was not upregulated, indicating that IRE1- and PERK-mediated pathways are involved in ER stress-dependent exosome release.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Serina-Treonina Quinases
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EIF-2 Quinase
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Endorribonucleases
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Exossomos
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Corpos Multivesiculares
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Estresse do Retículo Endoplasmático
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article