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Protein Imaging of CCN2 and CCN3 in Living Cells.
Hattori, Takako; Hoshijima, Mitsuhiro; Takigawa, Masaharu.
Afiliação
  • Hattori T; Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. hattori@md.okayama-u.ac.jp.
  • Hoshijima M; Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
  • Takigawa M; Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. takigawa@md.okayama-u.ac.jp.
Methods Mol Biol ; 1489: 211-215, 2017.
Article em En | MEDLINE | ID: mdl-27734379
ABSTRACT
Recent progress in molecular imaging technology has had a strong impact on improving our understanding of molecular translocation, receptor internalization, and interactions in living cells. The protocol in this chapter introduces an optimized technique for intracellular localization of CCN2 and CCN3 in live cells, one using GFP-tagged CCN2 and Halo-tagged CCN3.
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Base de dados: MEDLINE Assunto principal: Expressão Gênica / Fator de Crescimento do Tecido Conjuntivo / Proteína Sobre-Expressa em Nefroblastoma / Imagem Molecular Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Buscar no Google
Imprimir
XML
PubMed Links

Base de dados: MEDLINE Assunto principal: Expressão Gênica / Fator de Crescimento do Tecido Conjuntivo / Proteína Sobre-Expressa em Nefroblastoma / Imagem Molecular Idioma: En Ano de publicação: 2017 Tipo de documento: Article