Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1.
Nat Commun
; 7: 13097, 2016 10 14.
Article
em En
| MEDLINE
| ID: mdl-27739432
ABSTRACT
Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (mDA) neurons for cell replacement therapy for Parkinson's disease (PD). However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. To eliminate these unwanted cells, cell sorting using antibodies for specific markers such as CORIN or ALCAM has been developed, but neither marker is specific for ventral midbrain. Here we employ a double selection strategy for cells expressing both CORIN and LMX1AGFP, and report a cell surface marker to enrich mDA progenitors, LRTM1. When transplanted into 6-OHDA-lesioned rats, human iPSC-derived LRTM1+ cells survive and differentiate into mDA neurons in vivo, resulting in a significant improvement in motor behaviour without tumour formation. In addition, there was marked survival of mDA neurons following transplantation of LRTM1+ cells into the brain of an MPTP-treated monkey. Thus, LRTM1 may provide a tool for efficient and safe cell therapy for PD patients.
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1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
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Células-Tronco Pluripotentes Induzidas
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Neurônios Dopaminérgicos
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Células-Tronco Embrionárias Humanas
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article