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Decreased expression of miR­9 due to E50K OPTN mutation causes disruption of the expression of BDNF leading to RGC­5 cell apoptosis.
Jiang, Bo; Gao, Lin; Lei, Dawei; Liu, Jiannan; Shao, Zhengbo; Zhou, Xinrong; Li, Renke; Wu, Donglai; Xue, Fei; Zhu, Yuanmao; Yuan, Huiping.
Afiliação
  • Jiang B; Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
  • Gao L; Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
  • Lei D; Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
  • Liu J; Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
  • Shao Z; Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
  • Zhou X; Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
  • Li R; Division of Cardiovascular Surgery and Toronto General Research Institute, University Health Network and Department of Surgery, Division of Cardiac Surgery, University of Toronto, Toronto, ON M5G 1L7, Canada.
  • Wu D; The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150001, P.R. China.
  • Xue F; The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150001, P.R. China.
  • Zhu Y; The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150001, P.R. China.
  • Yuan H; Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Mol Med Rep ; 14(5): 4901-4905, 2016 Nov.
Article em En | MEDLINE | ID: mdl-27748809
The aims of the present study were to investigate the effect of E50K optineurin (OPTN) mutation on RGC­5 cells and to define the role of microRNA­9 (miR­9) in this system. Transfected RGC­5 cells were used to evaluate the effects of E50K OPTN on the expression of miR­9 and subsequent disruption of RGC­5 cell apoptosis was analyzed using western blotting. The results showed that the expression of E50K OPTN was associated with a marked reduction in the levels of miR­9 in the E50K OPTN­transfected RGC­5 cells. The E50K OPTN­dependent reductions in miR­9 led to increased expression of the transcriptional repressor, RE1­silencing transcription factor and decreased the expression of brain­derived neurotrophic factor. Thus, E50K OPTN may disrupt the expression of miR­9, suggesting a potential mechanism by which E50K OPTN mutation may lead to RGC­5 cell apoptosis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regulação Neoplásica da Expressão Gênica / Apoptose / Fator Neurotrófico Derivado do Encéfalo / Fator de Transcrição TFIIIA / MicroRNAs / Mutação / Neoplasias Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regulação Neoplásica da Expressão Gênica / Apoptose / Fator Neurotrófico Derivado do Encéfalo / Fator de Transcrição TFIIIA / MicroRNAs / Mutação / Neoplasias Idioma: En Ano de publicação: 2016 Tipo de documento: Article