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Differential expression of ISG 15 mRNA in peripheral blood mononuclear cells of nulliparous and multiparous pregnant versus non-pregnant Bos indicus cattle.
Soumya, N P; Das, D N; Jeyakumar, S; Mondal, S; Mor, A; Mundhe, U T.
Afiliação
  • Soumya NP; Genetics Lab, Dairy Production Section, ICAR-National Dairy Research Institute, Southern Regional Station, Bengaluru, India.
  • Das DN; Genetics Lab, Dairy Production Section, ICAR-National Dairy Research Institute, Southern Regional Station, Bengaluru, India.
  • Jeyakumar S; Livestock Research Centre, ICAR-National Dairy Research Institute, Southern Regional Station, Bengaluru, India.
  • Mondal S; ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, India.
  • Mor A; ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, India.
  • Mundhe UT; Genetics Lab, ICAR-National Dairy Research Institute, Bengaluru, India.
Reprod Domest Anim ; 52(1): 97-106, 2017 Feb.
Article em En | MEDLINE | ID: mdl-27766692
ABSTRACT
Embryonic mortality is found to be the main source of reproductive wastage in domestic ruminants. Many genes are involved in the growth and development of the embryo, and the interferon-stimulated gene 15 (ISG 15) is one of the major gene stimulated by interferon tau, the maternal recognition of pregnancy signal in ruminants. In this study, both genomic and cDNA sequences of ISG 15 from Bos indicus (Deoni breed) were amplified and characterized. The genomic sequence of Deoni ISG 15 exhibited 99% identity with Bos taurus and 97% identity with that of Bos mutus and Bubalus bubalis. Moreover qRT-PCR analysis revealed constitutive expression of the ISG 15 mRNA in peripheral blood mononuclear cells of Deoni heifers and multiparous cows during early pregnancy. Fourteen Deoni heifers and fifteen multiparous Deoni cows were synchronized for timed AI by CIDR-Ovsynch protocol, and six animals were kept as cyclic control in each group. Blood samples were collected on days 7, 14, 16, 18, 21, 30 and 45 from the day of AI. Pregnancy was confirmed by plasma progesterone level through ELISA. A significantly higher expression of ISG 15 mRNA was found on day 16 (p < .05) and day 18 (p < .05) of pregnancy in nulliparous heifers. Although in multiparous Deoni cows ISG 15 expression was greater in pregnant cows, difference was statistically non-significant. The result of this study indicates that ISG 15 gene expression is upregulated during 16-18 days of pregnancy and could be used as an early pregnancy marker in dairy cows especially in heifers.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Bovinos / Inseminação Artificial / Ubiquitinas / Citocinas / Sincronização do Estro Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Bovinos / Inseminação Artificial / Ubiquitinas / Citocinas / Sincronização do Estro Idioma: En Ano de publicação: 2017 Tipo de documento: Article