Ribosome-stalk biogenesis is coupled with recruitment of nuclear-export factor to the nascent 60S subunit.
Nat Struct Mol Biol
; 23(12): 1074-1082, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27775710
ABSTRACT
Nuclear export of preribosomal subunits is a key step during eukaryotic ribosome formation. To efficiently pass through the FG-repeat meshwork of the nuclear pore complex, the large pre-60S subunit requires several export factors. Here we describe the mechanism of recruitment of the Saccharomyces cerevisiae RNA-export receptor Mex67-Mtr2 to the pre-60S subunit at the proper time. Mex67-Mtr2 binds at the premature ribosomal-stalk region, which later during translation serves as a binding platform for translational GTPases on the mature ribosome. The assembly factor Mrt4, a structural homolog of cytoplasmic-stalk protein P0, masks this site, thus preventing untimely recruitment of Mex67-Mtr2 to nuclear pre-60S particles. Subsequently, Yvh1 triggers Mrt4 release in the nucleus, thereby creating a narrow time window for Mex67-Mtr2 association at this site and facilitating nuclear export of the large subunit. Thus, a spatiotemporal mark on the ribosomal stalk controls the recruitment of an RNA-export receptor to the nascent 60S subunit.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
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Saccharomyces cerevisiae
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Proteínas Nucleares
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Proteínas de Ligação a RNA
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Proteínas de Transporte Nucleocitoplasmático
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Proteínas de Saccharomyces cerevisiae
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Subunidades Ribossômicas Maiores
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article