bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring.
Elife
; 52016 10 28.
Article
em En
| MEDLINE
| ID: mdl-27791980
ABSTRACT
bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
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Ribonucleoproteínas
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RNA Mensageiro
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Transativadores
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Dineínas
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Proteínas de Homeodomínio
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Drosophila
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article