Emerging Methods in Chemoproteomics with Relevance to Drug Discovery.
Methods Mol Biol
; 1513: 11-22, 2017.
Article
em En
| MEDLINE
| ID: mdl-27807827
ABSTRACT
A powerful interplay exists between the recognition of gene families, sensitive techniques in proteomics, and the interrogation of protein function using chemical probes. The most prominent methods, such as affinity capture, activity-based protein profiling and photoaffinity labeling, are extensively reviewed in the literature. Here we briefly review additional methods developed in the past 15 years. These include "stability proteomics" methods such as proteomically analyzed cellular thermal shift assays and the use of chemical oxidation as a probe of structure, the use of multiple bead-linked kinase inhibitors to analyze inhibitor specificities, and advances in the use of proteolysis-targeting chimeras for selective protein elimination.
Palavras-chave
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas Quinases
/
Proteômica
/
Inibidores de Proteínas Quinases
/
Descoberta de Drogas
/
Proteínas de Neoplasias
/
Antineoplásicos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article