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Regulation of dendritic cell function by insulin/IGF-1/PI3K/Akt signaling through klotho expression.
Xuan, Nguyen Thi; Hoang, Nguyen Huy; Nhung, Vu Phuong; Duong, Nguyen Thuy; Ha, Nguyen Hai; Hai, Nong Van.
Afiliação
  • Xuan NT; a Institute of Genome Research, Vietnam Academy of Science and Technology , Cau Giay , Ha Noi , Vietnam.
  • Hoang NH; a Institute of Genome Research, Vietnam Academy of Science and Technology , Cau Giay , Ha Noi , Vietnam.
  • Nhung VP; a Institute of Genome Research, Vietnam Academy of Science and Technology , Cau Giay , Ha Noi , Vietnam.
  • Duong NT; a Institute of Genome Research, Vietnam Academy of Science and Technology , Cau Giay , Ha Noi , Vietnam.
  • Ha NH; a Institute of Genome Research, Vietnam Academy of Science and Technology , Cau Giay , Ha Noi , Vietnam.
  • Hai NV; a Institute of Genome Research, Vietnam Academy of Science and Technology , Cau Giay , Ha Noi , Vietnam.
J Recept Signal Transduct Res ; 37(3): 297-303, 2017 Jun.
Article em En | MEDLINE | ID: mdl-27808000
ABSTRACT
Insulin or insulin-like growth factor 1 (IGF-1) promotes the activation of phosphoinositide 3 kinase (PI3K)/Akt signaling in immune cells including dendritic cells (DCs), the most potent professional antigen-presenting cells for naive T cells. Klotho, an anti-aging protein, participates in the regulation of the PI3K/Akt signaling, thus the Ca2+-dependent migration is reduced in klotho-deficient DCs. The present study explored the effects of insulin/IGF-1 on DC function through klotho expression. To this end, the mouse bone marrow cells were isolated and cultured with GM-CSF to attain bone marrow-derived DCs (BMDCs). Cells were treated with insulin or IGF-1 and followed by stimulating with lipopolysaccharides (LPS). Tumor necrosis factor (TNF)-α formation was examined by enzyme-linked immunosorbent assay (ELISA). Phagocytosis was analyzed by FITC-dextran uptake assay. The expression of klotho was determined by quantitative PCR, immunoprecipitation and western blotting. As a result, treatment of the cells with insulin/IGF-1 resulted in reducing the klotho expression as well as LPS-stimulated TNF-α release and increasing the FITC-dextran uptake but unaltering reactive oxygen species (ROS) production in BMDCs. The effects were abolished by using pharmacological inhibition of PI3K/Akt with LY294002 and paralleled by transfecting DCs with klotho siRNA. In conclusion, the regulation of klotho sensitive DC function by IGF-1 or insulin is mediated through PI3K/Akt signaling pathway in BMDCs.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Dendríticas / Fator de Crescimento Insulin-Like I / Proteína Oncogênica v-akt / Glucuronidase / Insulina Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Dendríticas / Fator de Crescimento Insulin-Like I / Proteína Oncogênica v-akt / Glucuronidase / Insulina Idioma: En Ano de publicação: 2017 Tipo de documento: Article