Serotonergic Suppression of Mouse Prefrontal Circuits Implicated in Task Attention.
eNeuro
; 3(5)2016.
Article
em En
| MEDLINE
| ID: mdl-27844060
ABSTRACT
Serotonin (5-HT) regulates attention by neurobiological mechanisms that are not well understood. Layer 6 (L6) pyramidal neurons of prefrontal cortex play an important role in attention and express 5-HT receptors, but the serotonergic modulation of this layer and its excitatory output is not known. Here, we performed whole-cell recordings and pharmacological manipulations in acute brain slices from wild-type and transgenic mice expressing either eGFP or eGFP-channelrhodopsin in prefrontal L6 pyramidal neurons. Excitatory circuits between L6 pyramidal neurons and L5 GABAergic interneurons, including a population of interneurons essential for task attention, were investigated using optogenetic techniques. Our experiments show that prefrontal L6 pyramidal neurons are subject to strong serotonergic inhibition and demonstrate direct 5-HT-sensitive connections between prefrontal L6 pyramidal neurons and two classes of L5 interneurons. This work helps to build a neurobiological framework to appreciate serotonergic disruption of task attention and yields insight into the disruptions of attention observed in psychiatric disorders with altered 5-HT receptors and signaling.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Atenção
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Serotonina
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Córtex Pré-Frontal
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Células Piramidais
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article