Assessing the effects of threonyl-tRNA synthetase on angiogenesis-related responses.
Methods
; 113: 132-138, 2017 01 15.
Article
em En
| MEDLINE
| ID: mdl-27847344
ABSTRACT
Several recent reports have found a connection between specific aminoacyl-tRNA synthetases and the regulation of angiogenesis. As this new area of research is explored, it is important to have reliable assays to assess the specific angiogenesis functions of these enzymes. This review provides information about specific in vitro and in vivo methods that were used to assess the angiogenic functions of threonyl-tRNA synthetase including endothelial cell migration and tube assays as well as chorioallantoic membrane and tumor vascularization assays. The theory and discussion include best methods of analysis and quantification along with the advantages and limitations of each type of assay.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
/
Treonina-tRNA Ligase
/
Bioensaio
/
Membrana Corioalantoide
/
Aminoacilação de RNA de Transferência
/
Inibidores Enzimáticos
/
Neovascularização Patológica
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article