Gastroprotective effect and chemical characterization of a polysaccharide fraction from leaves of Croton cajucara.
Int J Biol Macromol
; 95: 153-159, 2017 Feb.
Article
em En
| MEDLINE
| ID: mdl-27864055
ABSTRACT
Croton cajucara Benth. is a tree from the Amazon Forest, where it is known as sacaca. Its leaves and barks are used in medicinal preparations to treat different diseases, including gastric ulcers. The crude polysaccharide fraction (CCP), obtained from the hot aqueous extract of C. cajucara leaves, was able to promote gastroprotection on an ethanol induced gastric ulcer model. Therefore, a bioguided fractionation was performed to isolate the active polysaccharide fraction. After freezing-thawing, ultrafiltration and dialyses at 100, 50, and 25kDa cut-off membranes, fraction 25R was obtained. It contained glucose, galactose, rhamnose, arabinose, galacturonic acid and mannose in a 755311 molar ratio approximately, and had a Mw of 42,840g/mol. Methylation analysis and NMR spectroscopy indicated that 25R is a very complex polysaccharide fraction containing type I rhamnogalacturonan, arabinan, type I arabinogalactan, type II arabinogalactan, rhamnan, starch and mannan. It was able to reduce ethanol-induced gastric ulcers in rats, through preservation of mucus and GSH levels.
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Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Estômago
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Folhas de Planta
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Citoproteção
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Croton
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article