Thyroid hormone status defines brown adipose tissue activity and browning of white adipose tissues in mice.
Sci Rep
; 6: 38124, 2016 12 12.
Article
em En
| MEDLINE
| ID: mdl-27941950
ABSTRACT
The present study aimed to determine the effect of thyroid hormone dysfunction on brown adipose tissue activity and white adipose tissue browning in mice. Twenty randomized female C57BL/6NTac mice per treatment group housed at room temperature were rendered hypothyroid or hyperthyroid. In-vivo small animal 18F-FDG PET/MRI was performed to determine the effects of hypo- and hyperthyroidism on BAT mass and BAT activity. Ex-vivo14C-acetate loading assay and assessment of thermogenic gene and protein expression permitted analysis of oxidative and thermogenic capacities of WAT and BAT of eu-, hyper and hypothyroid mice. 18F-FDG PET/MRI revealed a lack of brown adipose tissue activity in hypothyroid mice, whereas hyperthyroid mice displayed increased BAT mass alongside enhanced 18F-FDG uptake. In white adipose tissue of both, hyper- and hypothyroid mice, we found a significant induction of thermogenic genes together with multilocular adipocytes expressing UCP1. Taken together, these results suggest that both the hyperthyroid and hypothyroid state stimulate WAT thermogenesis most likely as a consequence of enhanced adrenergic signaling or compensation for impaired BAT function, respectively.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tecido Adiposo Marrom
/
Tecido Adiposo Branco
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Hipertireoidismo
/
Hipotireoidismo
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article