Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics.
Sci Rep
; 6: 38915, 2016 12 12.
Article
em En
| MEDLINE
| ID: mdl-27941956
ABSTRACT
Composting is a promising source of new organisms and thermostable enzymes that may be helpful in environmental management and industrial processes. Here we present results of metagenomic- and metatranscriptomic-based analyses of a large composting operation in the São Paulo Zoo Park. This composting exhibits a sustained thermophilic profile (50 °C to 75 °C), which seems to preclude fungal activity. The main novelty of our study is the combination of time-series sampling with shotgun DNA, 16S rRNA gene amplicon, and metatranscriptome high-throughput sequencing, enabling an unprecedented detailed view of microbial community structure, dynamics, and function in this ecosystem. The time-series data showed that the turning procedure has a strong impact on the compost microbiota, restoring to a certain extent the population profile seen at the beginning of the process; and that lignocellulosic biomass deconstruction occurs synergistically and sequentially, with hemicellulose being degraded preferentially to cellulose and lignin. Moreover, our sequencing data allowed near-complete genome reconstruction of five bacterial species previously found in biomass-degrading environments and of a novel biodegrading bacterial species, likely a new genus in the order Bacillales. The data and analyses provided are a rich source for additional investigations of thermophilic composting microbiology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Microbiologia do Solo
/
Compostagem
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Consórcios Microbianos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article