Evaluation of the performance of the IQ-Check kits and the USDA Microbiology Laboratory Guidebook methods for detection of Shiga toxin-producing Escherichia coli (STEC) and STEC and Salmonella simultaneously in ground beef.
J Appl Microbiol
; 122(3): 809-816, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-27987365
ABSTRACT
AIMS:
To evaluate the performance of the IQ-Check kits and the USDA Microbiology Laboratory Guidebook (MLG) methods for detection of the top seven Shiga toxin-producing Escherichia coli (STEC) (O157H7, O26, O45, O103, O111, O121 and O145) in ground beef and both STEC and Salmonella in co-inoculated samples. METHODS ANDRESULTS:
Ground beef samples inoculated with ~10 CFU of STEC or both STEC and Salmonella Typhimurium were stored at 4°C for 72 h, followed by screening with the IQ-Check and BAX System kit (MLG) methods that employ different enrichment media. STEC and S. Typhimurium were detected after 12 and 18 h and their presence was confirmed by colony isolation.CONCLUSIONS:
Both methods were able to detect STEC in ground beef after 12 h of enrichment in samples inoculated with low levels of the pathogen. STEC and S. Typhimurium can be detected and isolated in co-inoculated ground beef samples. SIGNIFICANCE AND IMPACT OF THE STUDY The IQ-Check methods are comparable to the MLG methods for detection of STEC and simultaneous detection of STEC and S. Typhimurium in seeded ground beef after a short enrichment time, thus the IQ-Check method can be useful for the food industry for rapid detection of these pathogens.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Salmonella
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Reação em Cadeia da Polimerase
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United States Department of Agriculture
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Escherichia coli Shiga Toxigênica
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Carne Vermelha
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article