Biodegradation of the endocrine disrupter 4-tert-octylphenol by the yeast strain Candida rugopelliculosa RRKY5 via phenolic ring hydroxylation and alkyl chain oxidation pathways.
Bioresour Technol
; 226: 55-64, 2017 Feb.
Article
em En
| MEDLINE
| ID: mdl-27987401
ABSTRACT
4-(1,1,3,3-tetramethylbutane)-phenol (4-tert-OP) is one of the most prevalent endocrine disrupting pollutants. Information about bioremediation of 4-tert-OP remains limited, and no study has been reported on the mechanism of 4-tert-OP degradation by yeasts. The yeast Candida rugopelliculosa RRKY5 was proved to be able to utilize 4-methylphenol, bisphenol A, 4-ethylphenol, 4-tert-butylphenol, 4-tert-OP, 4-tert-nonylphenol, isooctane, and phenol under aerobic conditions. The optimum conditions for 4-tert-OP degradation were 30°C, pH 5.0, and an initial 4-tert-OP concentration of 30mgL-1; the maximum biodegradation rate constant was 0.107d-1, equivalent to a minimum half-life of 9.6d. Scanning electron microscopy revealed formation of arthroconidia when cells were grown in the presence of 4-tert-OP, whereas the cells remained in the budding form without 4-tert-OP. Identification of the 4-tert-OP degradation metabolites using liquid chromatography-hybrid mass spectrometry revealed three different mechanisms via both branched alkyl side chain and aromatic ring cleavage pathways.
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Base de dados:
MEDLINE
Assunto principal:
Fenóis
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Candida
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Disruptores Endócrinos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article