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Assessment and Optimization of the GeneXpert Diagnostic Platform for Detection of Ebola Virus RNA in Seminal Fluid.
Pettitt, James; Higgs, Elizabeth; Fallah, Mosoka; Nason, Martha; Stavale, Eric; Marchand, Jonathan; Reilly, Cavan; Jensen, Kenneth; Dighero-Kemp, Bonnie; Tuznik, Kaylie; Logue, James; Bolay, Fatorma; Hensley, Lisa.
Afiliação
  • Pettitt J; Integrated Research Facility, National Institute of Allergy and Infectious Diseases, Fort Detrick, MD, USA.
  • Higgs E; Division of Clinical Research, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
  • Fallah M; Ministry of Health and Welfare, Monrovia, Liberia.
  • Nason M; Biostatistics Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, Bethesda, USA.
  • Stavale E; Integrated Research Facility, National Institute of Allergy and Infectious Diseases, Fort Detrick, MD, USA.
  • Marchand J; Integrated Research Facility, National Institute of Allergy and Infectious Diseases, Fort Detrick, MD, USA.
  • Reilly C; Division of Biostatistics, University of Minnesota School of Public Health, Minnesota, USA.
  • Jensen K; Integrated Research Facility, National Institute of Allergy and Infectious Diseases, Fort Detrick, MD, USA.
  • Dighero-Kemp B; Integrated Research Facility, National Institute of Allergy and Infectious Diseases, Fort Detrick, MD, USA.
  • Tuznik K; Integrated Research Facility, National Institute of Allergy and Infectious Diseases, Fort Detrick, MD, USA.
  • Logue J; Integrated Research Facility, National Institute of Allergy and Infectious Diseases, Fort Detrick, MD, USA.
  • Bolay F; Liberian Institute of Biomedical Research, Charlesville, Liberia.
  • Hensley L; Integrated Research Facility, National Institute of Allergy and Infectious Diseases, Fort Detrick, MD, USA.
J Infect Dis ; 215(4): 547-553, 2017 02 15.
Article em En | MEDLINE | ID: mdl-28003349
ABSTRACT
Recent studies have suggested that Ebola virus (EBOV) ribonucleic acid (RNA) potentially present in the semen of a large number of survivors of Ebola virus disease (EVD) in Western Africa may contribute to sexual transmission of EVD and generate new clusters of cases in regions previously declared EVD-free. These findings drive the immediate need for a reliable, rapid, user-friendly assay for detection of EBOV RNA in semen that is deployable to multiple sites across Western Africa. In this study, we optimized the Xpert EBOV assay for semen samples by adding dithiothreitol. Compared to the assays currently in use in Liberia (including Ebola Zaire Target 1, major groove binder real-time-polymerase chain reaction assays, and original Xpert EBOV assay), the modified Xpert EBOV assay demonstrated greater sensitivity than the comparator assays. Thus, the modified Xpert EBOV assay is optimal for large-scale monitoring of EBOV RNA persistence in male survivors.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sêmen / RNA Viral / Doença pelo Vírus Ebola / Ebolavirus / Reação em Cadeia da Polimerase em Tempo Real Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sêmen / RNA Viral / Doença pelo Vírus Ebola / Ebolavirus / Reação em Cadeia da Polimerase em Tempo Real Idioma: En Ano de publicação: 2017 Tipo de documento: Article