[hnRNP K Contributes to Adriamycin Resistance in Acute Myeloid Leukemia through Regulating Autophagy].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 24(6): 1665-1669, 2016 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-28024474
ABSTRACT
OBJECTIVE:
To explore the role of heterogeneous nuclear ribonucleoprotein(hnRNP) K regulating autophagy in the drug resistance of acute myeloid leukemia, so as to provide a new molecular marker for treatment of leukemia.METHODS:
The relationship between the expression level of hnRNP K and the drug resistance of myeloid leukemia was verified by fluorescence quantitative PCR; the expression of autophagy related protein LC3I/ II was detected by Western blot after the hnRNP K was modulated by RNA interference technology; the sensitivity of leukemia cells to doxorubicin was analyzed before and after the expression of hnRNP K were modulatd.RESULTS:
The expression of hnRNP K and LC3I/II significantly increased in bone marrow nonremission patients and in drug resistant cell line, however, the expression of LC3I/ II decreased when the expression of hnRNP K were reduced, while the sensitivity of cells to adriamycin could be recovered.CONCLUSION:
hnRNP K may be involved in the formation of adriamycin resistance in acute myeloid leukemia by regulating autophagy.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Leucemia Mieloide Aguda
Idioma:
Zh
Ano de publicação:
2016
Tipo de documento:
Article