In vitro control of parasitic nematodes of small ruminants using some plant species containing flavonoids.
Trop Anim Health Prod
; 49(2): 375-382, 2017 Feb.
Article
em En
| MEDLINE
| ID: mdl-28028635
ABSTRACT
This study determined in vitro anthelmintic efficacy of three plant species Trema orientalis, Urtica dioica and Zanthozylum capense on nematode larvae of small ruminants. Dried leaf samples (40 g) were extracted in 70% ethanol, in portions of 10 g and concentrated to 100 ml. Half and one quarter of the original crude extract were both made to 100 ml. Rectal faecal material from 10 Merino sheep and 25 Nguni goats was pooled within species and thoroughly hand-mixed. Dung samples, each of 5 g were cultured for 12 days at 27 °C. On day 13, 4 plates were watered and 4 others treated with ethanol to correct for solvent effect on mortality. The design was 2 (animal species) × 3 (plant species) × 3 (extract concentrations). In each of three runs, three plates were treated with each crude extract in three incremental concentrations. Surviving L3 larvae were isolated, counted and mortalities became indices of anthelmintic efficacy. Data from nematode larval mortality were analysed to determine the effect of animal species, plant species, concentration and their interactions. Efficacy was affected by concentration (P = 0.0001), animal species (P = 0.0046), plant species (P = 0.0572), the interactions of animal species and concentration (P = 0.0010), plant species and concentration (P = 0.0123) and concentration × animal × plant species (P = 0.0435).
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Base de dados:
MEDLINE
Assunto principal:
Doenças dos Ovinos
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Extratos Vegetais
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Doenças das Cabras
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Infecções por Nematoides
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Antinematódeos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article