Phospholipid flippases DnfA and DnfB exhibit differential dynamics within the A. nidulans Spitzenkörper.
Fungal Genet Biol
; 99: 26-28, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-28034798
ABSTRACT
The Spitzenkörper is a structure at the apex of growing cells in many filamentous fungi. Ultrastructural studies indicate that the Spitzenkörper is an organized mass of secretory vesicles, with different types of vesicles present in outer and inner layers. Here, we used live-cell imaging to demonstrate that the phospholipid flippases DnfA and DnfB, which preferentially localize to the outer and inner layers, respectively, exhibit different dynamics in the Spitzenkörper of Aspergillus nidulans. Additionally, deletion of dnfA partially destabilized the Spitzenkörper, while the depletion of cdc50, an essential ß-subunit of most flippases, had dramatic effects on hyphal tip organization and morphology.
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MEDLINE
Assunto principal:
Aspergillus nidulans
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Proteínas Fúngicas
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Hifas
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Proteínas de Transferência de Fosfolipídeos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article