Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses.
J Chromatogr A
; 1483: 101-109, 2017 Feb 03.
Article
em En
| MEDLINE
| ID: mdl-28043689
ABSTRACT
Suppressing the background interferences and enhancing the analytes signals are long-term goals in high performance electrospray ionization mass spectrometry (ESI-MS) analyses. We observed that performing electrospray in the presence of a concentrated acetonitrile atmosphere suppresses background interferences and enhances peptide signals. An enclosed nanoESI source was utilized to provide a stable atmosphere of concentrated acetonitrile vapor for high performance ESI-MS analyses. The median MS signal intensity increased by 5 times for a set of 23 BSA tryptic peptides in direct ESI-MS analysis. Further, the number of reproducibly and precisely quantified peptides could be improved 67% in six replicate label-free quantitative proteome analyses by this strategy.
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Base de dados:
MEDLINE
Assunto principal:
Acetonitrilas
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Espectrometria de Massas por Ionização por Electrospray
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Proteômica
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article