Glutamicibacter halophytocola sp. nov., an endophytic actinomycete isolated from the roots of a coastal halophyte, Limonium sinense.
Int J Syst Evol Microbiol
; 67(5): 1120-1125, 2017 May.
Article
em En
| MEDLINE
| ID: mdl-28056223
ABSTRACT
A novel actinobacterium, designated KLBMP 5180T, was isolated from the surface-sterilized root of a coastal halophyte, Limonium sinense, collected from the city of Lianyungang, Jiangsu Province, eastern China. The isolate was Gram-stain-positive, aerobic and non-motile. The components of the cell-wall peptidoglycan were lysine, glutamic acid and alanine. The predominant menaquinone was MK-9. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phospholipid, one unidentified glycolipid and two unidentified lipids. anteiso-C15ââ0 and iso-C16ââ0 were the major cellular fatty acids. The DNA G+C content of strain KLBMP 5180T was 60.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KLBMP 5180T belongs to the genus Glutamicibacter and was related most closely to Glutamicibacter nicotianae DSM 20123T (99.3â% similarity), Glutamicibacterarilaitensis Re117T (99.3â%) and Glutamicibacter mysorens LMG 16219T (99.1â%); similarity to other type strains of the genus Glutamicibacter was lower than 98.5â%. However, DNA-DNA relatedness values between strain KLBMP 5180T, G . nicotianae DSM 20123T, G. arilaitensis Re117T and G. mysorens LMG 16219T were 47.5±2.6, 51.3±3.1 and 41.2±4.3â%, respectively. The combination of DNA-DNA hybridization, phylogenetic, phenotypic and chemotaxonomic data supported the suggestion that strain KLBMP 5180T represents a novel species of the genus Glutamicibacter, for which the name Glutamicibacterhalophytocola sp. nov. is proposed. The type strain is KLBMP 5180T (=DSM 101718T=KCTC 39692T).
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Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Raízes de Plantas
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Plumbaginaceae
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Plantas Tolerantes a Sal
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Micrococcaceae
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article