Automated Antibody De Novo Sequencing and Its Utility in Biopharmaceutical Discovery.
J Am Soc Mass Spectrom
; 28(5): 803-810, 2017 05.
Article
em En
| MEDLINE
| ID: mdl-28105549
ABSTRACT
Applications of antibody de novo sequencing in the biopharmaceutical industry range from the discovery of new antibody drug candidates to identifying reagents for research and determining the primary structure of innovator products for biosimilar development. When murine, phage display, or patient-derived monoclonal antibodies against a target of interest are available, but the cDNA or the original cell line is not, de novo protein sequencing is required to humanize and recombinantly express these antibodies, followed by in vitro and in vivo testing for functional validation. Availability of fully automated software tools for monoclonal antibody de novo sequencing enables efficient and routine analysis. Here, we present a novel method to automatically de novo sequence antibodies using mass spectrometry and the Supernovo software. The robustness of the algorithm is demonstrated through a series of stress tests. Graphical Abstract á
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Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência de Proteína
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Descoberta de Drogas
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Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article