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Overexpression of a three-gene conidial pigment biosynthetic pathway in Aspergillus nidulans reveals the first NRPS known to acetylate tryptophan.
Sung, Calvin T; Chang, Shu-Lin; Entwistle, Ruth; Ahn, Green; Lin, Tzu-Shyang; Petrova, Vessela; Yeh, Hsu-Hua; Praseuth, Mike B; Chiang, Yi-Ming; Oakley, Berl R; Wang, Clay C C.
Afiliação
  • Sung CT; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA. Electronic address: calvin.sung@medsch.ucr.edu.
  • Chang SL; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA; Drug Discovery and Development Center, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan, ROC. Electronic address: slchang1120@mail.cnu.edu.tw.
  • Entwistle R; Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA. Electronic address: raentw@ku.edu.
  • Ahn G; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA. Electronic address: green8ahn@gmail.com.
  • Lin TS; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA. Electronic address: tzushyang.lin@usc.edu.
  • Petrova V; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA. Electronic address: petrovavessela@gmail.com.
  • Yeh HH; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA; Drug Discovery and Development Center, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan, ROC. Electronic address: ysh521@mail.cnu.edu.tw.
  • Praseuth MB; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA. Electronic address: mpraseuth@gmail.com.
  • Chiang YM; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA; Department of Pharmacy, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan, ROC. Electronic address: ymchiang@mail.cnu.edu.tw.
  • Oakley BR; Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA. Electronic address: boakley@ku.edu.
  • Wang CCC; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA; Department of Chemistry, Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, CA 90089, USA. Electronic address: clay
Fungal Genet Biol ; 101: 1-6, 2017 04.
Article em En | MEDLINE | ID: mdl-28108400
ABSTRACT
Fungal nonribosomal peptide synthetases (NRPSs) are megasynthetases that produce cyclic and acyclic peptides. In Aspergillus nidulans, the NRPS ivoA (AN10576) has been associated with the biosynthesis of grey-brown conidiophore pigments. Another gene, ivoB (AN0231), has been demonstrated to be an N-acetyl-6-hydroxytryptophan oxidase that putatively acts downstream of IvoA. A third gene, ivoC, has also been predicted to be involved in pigment biosynthesis based on publicly available genomic and transcriptomic information. In this paper, we report the replacement of the promoters of the ivoA, ivoB, and ivoC genes with the inducible promoter alcA in a single cotransformation. Co-overexpression of the three genes resulted in the production of a dark-brown pigment in hyphae. In addition, overexpression of each of the Ivo genes, ivoA-C, individually or in combination, allowed us to isolate intermediates and confirm the function of each gene. IvoA was found to be the first known NRPS to carry out the acetylation of the amino acid, tryptophan.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeo Sintases / Pigmentação / Monofenol Mono-Oxigenase / Biossíntese de Peptídeos Independentes de Ácido Nucleico Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeo Sintases / Pigmentação / Monofenol Mono-Oxigenase / Biossíntese de Peptídeos Independentes de Ácido Nucleico Idioma: En Ano de publicação: 2017 Tipo de documento: Article