TGFß/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands.
Open Biol
; 7(1)2017 01.
Article
em En
| MEDLINE
| ID: mdl-28123053
ABSTRACT
Signalling by TGFß superfamily factors plays an important role in tissue growth and cell proliferation. In Drosophila, the activity of the TGFß/Activin signalling branch has been linked to the regulation of cell growth and proliferation, but the cellular and molecular basis for these functions are not fully understood. In this study, we show that both the RII receptor Punt (Put) and the R-Smad Smad2 are strongly required for cell and tissue growth. Knocking down the expression of Put or Smad2 in salivary glands causes alterations in nucleolar structure and functions. Cells with decreased TGFß/Activin signalling accumulate intermediate pre-rRNA transcripts containing internal transcribed spacer 1 regions accompanied by the nucleolar retention of ribosomal proteins. Thus, our results show that TGFß/Activin signalling is required for ribosomal biogenesis, a key aspect of cellular growth control. Importantly, overexpression of Put enhanced cell growth induced by Drosophila Myc, a well-characterized inducer of nucleolar hypertrophy and ribosome biogenesis.
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Base de dados:
MEDLINE
Assunto principal:
Ribossomos
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Glândulas Salivares
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Receptores de Activinas Tipo II
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Proteínas de Drosophila
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Drosophila melanogaster
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Proteína Smad2
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article