Detection of vulnerable neurons damaged by environmental insults in utero.
Proc Natl Acad Sci U S A
; 114(9): 2367-2372, 2017 02 28.
Article
em En
| MEDLINE
| ID: mdl-28123061
ABSTRACT
Development of prognostic biomarkers for the detection of prenatally damaged neurons before manifestations of postnatal disorders is an essential step for prevention and treatment of susceptible individuals. We have developed a versatile fluorescence reporter system in mice enabling detection of Heat Shock Factor 1 activation in response to prenatal cellular damage caused by exposure to various harmful chemical or physical agents. Using an intrautero electroporation-mediated reporter assay and transgenic reporter mice, we are able to identify neurons that survive prenatal exposure to harmful agents but remain vulnerable in postnatal life. This system may provide a powerful tool for exploring the pathogenesis and treatment of multiple disorders caused by exposure to environmental stress before symptoms become manifested, exacerbated, and/or irreversible.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Efeitos Tardios da Exposição Pré-Natal
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Córtex Cerebral
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Elementos de Resposta
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Fatores de Transcrição de Choque Térmico
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Neurônios
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article