Reprogramming Postnatal Human Epidermal Keratinocytes Toward Functional Neural Crest Fates.
Stem Cells
; 35(5): 1402-1415, 2017 05.
Article
em En
| MEDLINE
| ID: mdl-28142205
ABSTRACT
During development, neural crest (NC) cells are induced by signaling events at the neural plate border of all vertebrate embryos. Initially arising within the central nervous system, NC cells subsequently undergo an epithelial to mesenchymal transition to migrate into the periphery, where they differentiate into diverse cell types. Here we provide evidence that postnatal human epidermal keratinocytes (KC), in response to fibroblast growth factor 2 and insulin like growth factor 1 signals, can be reprogrammed toward a NC fate. Genome-wide transcriptome analyses show that keratinocyte-derived NC cells are similar to those derived from human embryonic stem cells. Moreover, they give rise in vitro and in vivo to NC derivatives such as peripheral neurons, melanocytes, Schwann cells and mesenchymal cells (osteocytes, chondrocytes, adipocytes, and smooth muscle cells). By demonstrating that human keratin-14+ KC can form NC cells, even from clones of single cells, our results have important implications in stem cell biology and regenerative medicine. Stem Cells 2017;351402-1415.
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Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
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Linhagem da Célula
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Reprogramação Celular
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Células Epidérmicas
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Crista Neural
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article