Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling.
J Ind Microbiol Biotechnol
; 44(3): 413-417, 2017 03.
Article
em En
| MEDLINE
| ID: mdl-28160205
ABSTRACT
Heterologous protein production in the yeast Pichia pastoris can be limited by biological responses to high expression levels; the unfolded protein response (UPR) is a key determinant of the success of protein production in this organism. Here, we used untargeted NMR metabolic profiling (metabolomics) of a number of different recombinant strains, carried out in a miniaturized format suitable for screening-level experiments. We identified a number of metabolites (from both cell extracts and supernatants) which correlated well with UPR-relevant gene transcripts, and so could be potential biomarkers for future high-throughput screening of large numbers of P. pastoris clones.
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MEDLINE
Assunto principal:
Pichia
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Proteínas Recombinantes
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Metabolômica
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article