Enzymes for liberation of pantothenic acid in blood: use of plasma pantetheinase.
Am J Clin Nutr
; 50(5): 1072-8, 1989 Nov.
Article
em En
| MEDLINE
| ID: mdl-2816792
ABSTRACT
Reported normal concentrations for human whole-blood total pantothenic acid vary from 1.1 to 12 mumol/L. This wide range may partly arise from the various enzymes used for liberation of pantothenic acid from coenzyme A, particularly the source of pantetheinase. A purified pantetheinase from pig kidney had greater than 100 times the specific activity and less than 0.01 times the pantothenate content of other commonly used extracts. Endogenous pantetheinase activity in human plasma was identified (11.2 +/- 2.0 mumol pantothenate .min-1.L-1, n = 29) and found comparable to the activity usually added from exogenous sources for liberation of pantothenate from whole blood (1-13 mumol.min-1.L-1). Alkaline phosphatase alone liberated as much pantothenate from hemolyzed whole blood as did alkaline phosphatase with pantetheinase. Previous reports of total blood pantothenate may be elevated by pantothenate in the pantetheinase extracts, an unnecessary source of error. Whole-blood total pantothenate concentrations less than 4.6 mumol/L are normal and do not indicate deficiency, as is often currently quoted.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Pantotênico
/
Coenzima A
/
Fosfatase Alcalina
/
Amidoidrolases
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article