Isolation of Adipose-Derived Stromal Vascular Fraction Cells Using a Novel Point-of-Care Device: Cell Characterization and Review of the Literature.
Tissue Eng Part C Methods
; 23(3): 125-135, 2017 03.
Article
em En
| MEDLINE
| ID: mdl-28177263
ABSTRACT
The GID SVF-1 and GID SVF-2 are closed-system, disposable, scalable cellular isolation devices designed for isolating the human adipose-derived stromal vascular fraction (hSVF) from lipoaspirates at the clinical point of care. The purpose of this study was to characterize the device performance with respect to cell yield and viability of the hSVF, as well as the hSVF purity and cellular composition. Our results demonstrate that adipose-derived hSVF can be safely obtained using both devices and standardized methods, yielding cells that are free of bacterial contaminants as measured by endotoxin levels, Gram stain, and culture. In addition, they produce cellular preparations with compositions consistent with reported values within the peer-reviewed literature using similar methods.
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MEDLINE
Assunto principal:
Diferenciação Celular
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Separação Celular
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Tecido Adiposo
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Sistemas Automatizados de Assistência Junto ao Leito
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Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article